ABSTRACT
Many people are used medicinal plants due to natural, low risks and complications, and low costs as compared with the synthetic drugs. Incidence of drug resistance against chemical antimicrobial drugs has led the use of medicinal plants for treatment of infections in recent years. The aim of this study was to examine possible antibacterial effects of four herbal plants in vitro. Four species of domestic plants from Fars- Fasa [Iran] including Ziziphora, Stachys, Teucrium and Barberry were collected in spring and dried. Hydro-alcoholic extracts of these plants were prepared using standard methods. Antibacterial effects were examined with disk diffusion method and serial both dilution. To standardize of study, we used antibiogram disks and ATCC bacteria. Teucrium extract with 1/8 dilution and Barberry extract with 1/4 dilution showed antibacterial effects in serial dilution method. Moreover, by using the disk diffusion method, antibacterial effects of both mentioned extracts against E.coli ATCC 25922 and S.aureus ATCC 25923, were found as compared amoxicillin, ciprofloxacin, vancomycin and imipenem. The results of this study and other studies show that extracts of herbal plants, instead of chemical drugs, can be used to treat infections. Of course, before using them all their side effects should be carefully checked in vitro and in vivo studies
ABSTRACT
Prevalence of abortion is higher in women with autoimmune thyroid disease. In the majority of cases, however, no abnormality of thyroid function is detected despite the high levels of antithyroid antibodies. The direct influence of such harmful autoantibodies in female reproductive organs may serve a role in pregnancy loss. In this study, expression of thyroglobulin in the reproductive tissues of cycling mice has been evaluated. Stages of estrous cycle were determined by cellular morphology and ratio of epithelial cells to leukocytes in vaginal smear of Balb/C mice. At each phase, the mice were sacrificed and their uterus, ovary and fallopian tubes were removed. Expression of thyroglobulin-specific transcript in endometrium was investigated by two sets of primers using reverse transcriptase-polymerase chain reaction [RT-PCR]. In addition, expression of thyroglobulin in reproductive tissues was assessed by immunohistochemistry and dot blot analysis. The results showed that thyroglobulin mRNA is not expressed in endometrial tissue of Balb/C mice at any stage of estrous cycle. Immunohistochemical analysis also confirmed that thyroglobulin or its cross reactive-antigens are not expressed at the protein level in the female reproductive organs. The results showed that thyroglobulin was not expressed in the reproductive organs of female mice. It is plausible that antithyroglobulin antibodies could interact with newly-generated antigens during placentation and pregnancy